Fluorescence imaging is more than just capturing an image. Each fluorochrome
channel carries crucial information about the hybridization status of the DNA probe
kit used for hybridization. This is why Isis always provides full user control over the
image: each color channel can be visualized and enhanced individually with a few
simple steps. Original images remain present at all times, and various color display
modes offer the possibility to illustrate exactly the information that is required.
Isis supports up to 12 color channels and allows the user to easily switch between
different fluorochrome combinations. Global settings files (’experiment types’)
contain all necessary parameters required to adjust the system to another sample
- including the fluorochromes used, the automated image enhancement
algorithms, the look-up tables for false color display, and more.
Image acquisition is particularly simple with Isis. Each color channel is acquired
with automated, dynamic integration time adjustment, thus providing the best
signal conditions at anytime. Isis supports many motorized microscopes, enabling
effortless acquisition of even complex images with many color channels. And
extended focus images are of course automatically created from focus stacks.
One-click image acquisition of fluorescence images with up to
12 color channels based on automated integration time adjustment
Support of many motorized microscopes and filter wheels
Extended focus imaging (microscopes with motorized focus are supported)
Easy automated, or semi-automated, enhancement of images, individually
for each color channel, and with unlimited undo functionality
Local thresholding to generate homogeneously stained images
Powerful chromosome banding and signal enhancement filters
Full karyotyping functionality for FISH stained chromosomes (optional)
Single color channel display as color, grayscale, or inverted grayscale image
Various pseudo color display modes (modified color mode,
merged color mode, primary false color mode) with editable look-up tables
Comprehensive image annotation with unlimited colors and fonts
Various measurement functions including fluorochrome profile evaluation